Flow cytometric analysis of Tuberin expression in HepG2 cells using Tuberin antibody (Cat#68712, 1:2,000). Green, isotype control; Red, Tuberin.
Western blotting analysis using anti-Tuberin antibody (Cat#68712). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Tuberin antibody (Cat#89712, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Tuberin antibody (Cat#68712). Tuberin expression in wild type (WT) and Tuberin shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Tuberin antibody (Cat#68712, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Tuberin knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Tuberin antibody (Cat#68712, 1:2,000) and analyzed using BD flow cytometer.
