Flow cytometric analysis of DLAT expression in HepG2 cells using DLAT antibody (Cat#69417, 1:2,000). Green, isotype control; red, DLAT.
Immunocytochemical staining of HepG2 cells with DLAT antibody (Cat#69417, 1:1,000). Nuclei were stained blue with DAPI; DLAT was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-DLAT antibody (Cat#69417). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DLAT antibody (Cat#69417, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-DLAT antibody (Cat#69417). DLAT expression in wild type (WT) and DLAT shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with anti-DLAT antibody (Cat#69417, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of DLAT knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-DLAT antibody (Cat#69417, 1:2,000) and analyzed using CytoFLEX.
Immunocytochemical staining of HeLa cells using anti-DLAT antibody (Cat#69417, 1:1,000), Top panel: wild-type (WT); Bottom panal: DLAT shRNA knockdown (KD). Nuclei were stained blue with DAPI; DLAT was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
