• 一抗
  • 二抗
  • 慢病毒
  • 细胞系
  • 裂解物
  • 试剂类

KD-Validated Anti-Cyclin D1 Rabbit Monoclonal Antibody #69541

一键复制产品信息
Flow cytometric analysis of Cyclin D1 expression in C2C12 cells using Cyclin D1 antibody (Cat#69541, 1:2,000). Green, isotype control; red, Cyclin D1.
Immunocytochemical staining of C2C12 cells with Cyclin D1 antibody (Cat#69541, 1:1,000). Nuclei were stained blue with DAPI; Cyclin D1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Cyclin D1 antibody (Cat#69541). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cyclin D1 antibody (Cat#69541, 1:10,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Cyclin D1 antibody (Cat#69541). Cyclin D1 expression in wild type (WT) and Cyclin D1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Cyclin D1 antibody (Cat#69541, 1:10,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Cyclin D1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Cyclin D1 antibody (Cat#69541, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-Cyclin D1 antibody (Cat#69541, 1:1,000), Top panel: wild-type (WT); Bottom panal: Cyclin D1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Cyclin D1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
  • 基因名:
    CCND1
  • 货号:
    69541
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-Cyclin D1 Rabbit Monoclonal Antibody
Aliases CCND1; Cyclin D1; U21B31; PRAD1; BCL1; B-Cell Lymphoma 1 Protein; G1/S-Specific Cyclin-D1; B-Cell CLL/Lymphoma 1; BCL-1 Oncogene; PRAD1 Oncogene; D11S287E; Cyclin D1 (PRAD1: Parathyroid Adenomatosis 1); Parathyroid Adenomatosis 1; G1/S-Specific Cyclin D1; BCL-1
Background

Gene Name: CCND1

NCBI Gene Entry: 595

UniProt Entry: P24385

Application Information

Molecular Weight: Predicted, 34 kDa, observed, 34 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB735

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human Cyclin D1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Cyclin D1 expression in C2C12 cells using Cyclin D1 antibody (Cat#69541, 1:2,000). Green, isotype control; red, Cyclin D1.
  • Immunocytochemical staining of C2C12 cells with Cyclin D1 antibody (Cat#69541, 1:1,000). Nuclei were stained blue with DAPI; Cyclin D1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-Cyclin D1 antibody (Cat#69541). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cyclin D1 antibody (Cat#69541, 1:10,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-Cyclin D1 antibody (Cat#69541). Cyclin D1 expression in wild type (WT) and Cyclin D1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Cyclin D1 antibody (Cat#69541, 1:10,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Validation of Cyclin D1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Cyclin D1 antibody (Cat#69541, 1:2,000) and analyzed using BD flow cytometer.
  • Immunocytochemical staining of HeLa cells using anti-Cyclin D1 antibody (Cat#69541, 1:1,000), Top panel: wild-type (WT); Bottom panal: Cyclin D1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Cyclin D1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
Citation(0)
Flow cytometric analysis of Cyclin D1 expression in C2C12 cells using Cyclin D1 antibody (Cat#69541, 1:2,000). Green, isotype control; red, Cyclin D1.
Immunocytochemical staining of C2C12 cells with Cyclin D1 antibody (Cat#69541, 1:1,000). Nuclei were stained blue with DAPI; Cyclin D1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Cyclin D1 antibody (Cat#69541). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cyclin D1 antibody (Cat#69541, 1:10,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Cyclin D1 antibody (Cat#69541). Cyclin D1 expression in wild type (WT) and Cyclin D1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Cyclin D1 antibody (Cat#69541, 1:10,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Cyclin D1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Cyclin D1 antibody (Cat#69541, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-Cyclin D1 antibody (Cat#69541, 1:1,000), Top panel: wild-type (WT); Bottom panal: Cyclin D1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Cyclin D1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
  • 联系电话

    0551-63635518

    0551-65578998

  • 联系邮箱

    support@genuinbiotech.cn

    sales@genuinbiotech.cn

微信公众号

Copyright © 2025 合肥善本生物科技有限公司

All Rights Reserved. 皖ICP备2023000814号