Flow cytometric analysis of HIF-1 beta expression in HepG2 cells using anti-HIF-1 beta antibody (Cat#69657,1:2,000). Green, isotype control; red, HIF-1 beta.
Immunocytochemical staining of HepG2 cells with HIF-1 beta antibody (Cat#69657, 1:1,000). Nuclei were stained blue with DAPI; HIF-1 beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-HIF 1 β antibody (Cat#69657). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HIF 1 β antibody (Cat#69657,1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201,1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-HIF-1 beta antibody (Cat#69657).HIF-1 beta expression in wild type (WT) and HIF-1 beta shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-HIF-1 beta antibody (Cat#69657,1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201,1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
