Flow cytometric analysis of Cullin 1 expression in HepG2 cells using Cullin 1 antibody (Cat#69741, 1:2,000). Green, isotype control; red, Cullin 1.
Immunocytochemical staining of HT-1080 cells with Cullin 1 antibody (Cat#69741, 1:1,000). Nuclei were stained blue with DAPI; Cullin 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Cullin 1 antibody (Cat#69741). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cullin 1 antibody (Cat#69741, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Cullin 1 antibody (Cat#69741). Cullin 1 expression in wild type (WT) and Cullin 1 shRNA knockdown (KD) 293T cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Cullin 1 antibody (Cat#69741, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Cullin 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) 293T cells were stained with anti-Cullin 1 antibody (Cat#69741, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of 293T cells using anti-Cullin 1 antibody (Cat#69741, 1:1,000), Top panel: wild-type (WT); Bottom panal: Cullin 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Cullin 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
