Flow cytometric analysis of GSK3 alpha expression in HepG2 cells using GSK3 alpha antibody (Cat#69743, 1:2,000). Green, isotype control; red, GSK3 alpha.
Immunocytochemical staining of HepG2 cells with GSK3 alpha antibody (Cat#69743, 1:1,000). Nuclei were stained blue with DAPI; GSK3 alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-GSK3 α antibody (Cat#69743). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GSK3 α antibody (Cat#69743, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-GSK3 alpha antibody (Cat#69743). GSK3 alpha expression in wild type (WT) and GSK3 alpha shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-GSK3 alpha antibody (Cat#69743, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
