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KO-Validated Anti-CREB1 Mouse Monoclonal Antibody #71152

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Western blotting analysis using anti-CREB1 antibody (Cat#71152). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CREB1 antibody (Cat#71152, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). CREB1, cAMP responsive element binding protein 1.
Western blotting analysis using anti-CREB1 antibody (Cat#71152). CREB1 expression in wild type (WT) and CREB1 knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CREB1 antibody (Cat#71152, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    CREB1
  • 货号:
    71152
  • 应用:
    WB
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KO-Validated Anti-CREB1 Mouse Monoclonal Antibody
Aliases CREB1; CAMP Responsive Element Binding Protein 1; Cyclic AMP-Responsive Element-Binding Protein 1; CREB-1; Cyclic Adenosine 3',5'-Monophosphate Response Element-Binding Protein CREB; Cyclic Adenosine 3',5'-Monophosphate Response Element Binding Protein; CAMP-Responsive Element-Binding Protein 1; CAMP-Response Element-Binding Protein-1; Active Transcription Factor CREB; Transactivator Protein; CREB
Background

Gene Name: CREB1

NCBI Gene Entry: 1385

UniProt Entry: P16220

Application Information

Molecular Weight: Predicted, 35 kDa, observed, 43 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB565

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB)

Immunogen Recombinant protein of human CREB
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,500-1:5,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-CREB1 antibody (Cat#71152). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CREB1 antibody (Cat#71152, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). CREB1, cAMP responsive element binding protein 1.
  • Western blotting analysis using anti-CREB1 antibody (Cat#71152). CREB1 expression in wild type (WT) and CREB1 knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CREB1 antibody (Cat#71152, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Western blotting analysis using anti-CREB1 antibody (Cat#71152). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CREB1 antibody (Cat#71152, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). CREB1, cAMP responsive element binding protein 1.
Western blotting analysis using anti-CREB1 antibody (Cat#71152). CREB1 expression in wild type (WT) and CREB1 knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CREB1 antibody (Cat#71152, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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