Flow cytometric analysis of Syntaxin 17 expression in HepG2 cells using anti-Syntaxin 17 antibody (Cat#71159, 1:2,000) . Green, isotype control; red, Syntaxin 17.
Immunocytochemical staining of HepG2 cells with anti-Syntaxin 17 antibody (Cat#71159, 1:1,000) . Nuclei were stained blue with DAPI; Syntaxin 17 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-syntaxin 17 antibody (Cat#71159). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-syntaxin 17 antibody (Cat#71159, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-syntaxin 17 antibody (Cat#71159). Syntaxin 17 expression in wild-type (WT) and syntaxin 17 (STX17) knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-syntaxin 17 antibody (Cat#71159, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
