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KO-Validated Anti-14-3-3 alpha/beta Rabbit Monoclonal Antibody #71174

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Flow cytometric analysis of 14-3-3 alpha/beta expression in HepG2 cells using 14-3-3 alpha/beta antibody (Cat#71174, 1:2,000). Green, isotype control; red, 14-3-3 alpha/beta.
Immunocytochemical staining of HepG2 cells with anti-14-3-3 alpha/beta antibody (Cat#71174, 1:1,000). Nuclei were stained blue with DAPI; 14-3-3 alpha/beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-14-3-3 alpha/beta antibody (Cat#71174). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-14-3-3 alpha/beta antibody (Cat#71174, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-14-3-3 alpha/beta antibody (Cat#71174). 14-3-3 alpha/beta expression in wild-type (WT) and YWHAB knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-14-3-3 alpha/beta antibody (Cat#71174, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    YWHAB
  • 货号：
    71174
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KO-Validated Anti-14-3-3 alpha/beta Rabbit Monoclonal Antibody
Aliases YWHAB; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Beta; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Alpha Polypeptide; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Beta Polypeptide; 14-3-3 Protein Beta/Alpha; 14-3-3 Alpha; Protein 1054; KCIP-1; YWHAA; Protein Kinase C Inhibitor Protein-1; Protein Kinase C Inhibitor Protein 1; Epididymis Secretory Protein Li 1; 14-3-3 Beta; HEL-S-1; GW128; HS1
Background

Gene Name: YWHAB

NCBI Gene Entry: 7529

UniProt Entry: P31946
Application Information

Molecular Weight: Predicted, 28 kDa, observed, 28 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB2045

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human 14-3-3 alpha + beta
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of 14-3-3 alpha/beta expression in HepG2 cells using 14-3-3 alpha/beta antibody (Cat#71174, 1:2,000). Green, isotype control; red, 14-3-3 alpha/beta.
  • Immunocytochemical staining of HepG2 cells with anti-14-3-3 alpha/beta antibody (Cat#71174, 1:1,000). Nuclei were stained blue with DAPI; 14-3-3 alpha/beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-14-3-3 alpha/beta antibody (Cat#71174). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-14-3-3 alpha/beta antibody (Cat#71174, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-14-3-3 alpha/beta antibody (Cat#71174). 14-3-3 alpha/beta expression in wild-type (WT) and YWHAB knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-14-3-3 alpha/beta antibody (Cat#71174, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    YWHAB
  • 货号：
    71174
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of 14-3-3 alpha/beta expression in HepG2 cells using 14-3-3 alpha/beta antibody (Cat#71174, 1:2,000). Green, isotype control; red, 14-3-3 alpha/beta.
Immunocytochemical staining of HepG2 cells with anti-14-3-3 alpha/beta antibody (Cat#71174, 1:1,000). Nuclei were stained blue with DAPI; 14-3-3 alpha/beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-14-3-3 alpha/beta antibody (Cat#71174). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-14-3-3 alpha/beta antibody (Cat#71174, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-14-3-3 alpha/beta antibody (Cat#71174). 14-3-3 alpha/beta expression in wild-type (WT) and YWHAB knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-14-3-3 alpha/beta antibody (Cat#71174, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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