Flow cytometric analysis of Tripartite motif containing 28 expression in HT-1080 cells using Tripartite motif containing 28 antibody (Cat#71189, 1:2,000). Green, isotype control; red, Tripartite motif containing 28.
Immunocytochemical staining of HT-1080 cells with Tripartite motif containing 28 antibody (Cat#71189, 1:1,000). Nuclei were stained blue with DAPI; Tripartite motif containing 28 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-tripartite motif containing 28 antibody (Cat#71189). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-tripartite motif containing 28 antibody (Cat#71189, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-tripartite motif containing 28 antibody (Cat#71189). Tripartite motif containing 28 expression in wild type (WT) and tripartite motif containing 28 (TRIM28) knockout (KO) HSHC cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-tripartite motif containing 28 antibody (Cat#71189, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
