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KO-Validated Anti-Phospho-Histone H3 (T3) Rabbit Monoclonal Antibody #71194

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Immunocytochemical staining of C2C12 cells with anti-Phospho-Histone H3 (T3) antibody (Cat#71194, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Histone H3 (T3) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-Histone H3 (T3) antibody (Cat#71194). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-Histone H3 (T3) antibody (Cat#71194, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-phospho-histone H3 (T3) antibody (Cat#71194). Phospho-histone H3 (T3) expression in wild type (WT) and H3-3A knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-histone H3 (T3) antibody (Cat#71194, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    H3-3A
  • 货号：
    71194
  • 应用：
    WB, IC
  • 物种反应性：
    H, M
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KO-Validated Anti-Phospho-Histone H3 (T3) Rabbit Monoclonal Antibody
Aliases H3-3A; H3.3 Histone A; H3.3A; H3F3A; H3F3; H3 Histone Family Member 3A; H3 Histone, Family 3A; Histone H3.3; BRYLIB1; H3-3B; H3.3B; H3F3B
Background

Gene Name: H3-3A

NCBI Gene Entry: 3020

UniProt Entry: P84243
Application Information

Molecular Weight: Predicted, 15 kDa; observed, 16 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB2460

Species Reactivity: Human, mouse

Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Phospho-Histone H3 (T3)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Immunocytochemical staining of C2C12 cells with anti-Phospho-Histone H3 (T3) antibody (Cat#71194, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Histone H3 (T3) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
  • Western blotting analysis using anti-phospho-Histone H3 (T3) antibody (Cat#71194). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-Histone H3 (T3) antibody (Cat#71194, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-phospho-histone H3 (T3) antibody (Cat#71194). Phospho-histone H3 (T3) expression in wild type (WT) and H3-3A knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-histone H3 (T3) antibody (Cat#71194, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    H3-3A
  • 货号：
    71194
  • 应用：
    WB, IC
  • 物种反应性：
    H, M
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Immunocytochemical staining of C2C12 cells with anti-Phospho-Histone H3 (T3) antibody (Cat#71194, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Histone H3 (T3) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-Histone H3 (T3) antibody (Cat#71194). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-Histone H3 (T3) antibody (Cat#71194, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-phospho-histone H3 (T3) antibody (Cat#71194). Phospho-histone H3 (T3) expression in wild type (WT) and H3-3A knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-histone H3 (T3) antibody (Cat#71194, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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