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KO-Validated Anti-Phospho-Histone H2A.X (S139) Rabbit Monoclonal Antibody #71197

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Western blotting analysis using anti-phospho-Histone H2A.X (S139) antibody (Cat#71197). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-Histone H2A.X (S139) antibody (Cat#71197, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-phospho-Histone H2A.X (S139) antibody (Cat#71197). Phospho-Histone H2A.X (S139) expression in wild-type (WT) and H2AX knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-Histone H2A.X (S139) antibody (Cat#71197, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Phospho-Histone H2A.X (S139) expression in HepG2 cells using Phospho-Histone H2A.X (S139) antibody (Cat#71197, 1：2000). Green, isotype control; red, Phospho-Histone H2A.X (S139).
Immunocytochemical staining of HepG2 cells with anti-Phospho-Histone H2A.X (S139) antibody (Cat#71197, 1:1000) . Nuclei were stained blue with DAPI; Phospho-Histone H2A.X (S139) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • 基因名：
    H2AX
  • 货号：
    71197
  • 应用：
    WB
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KO-Validated Anti-Phospho-Histone H2A.X (S139) Rabbit Monoclonal Antibody
Aliases H2AX; H2A.X Variant Histone; H2AFX; H2A Histone Family Member X; Histone H2A.X; Histone H2AX; H2A Histone Family, Member X; H2AX Histone; H2A.X; H2A/X; H2a/X
Background

NCBI Gene Entry: 3014

UniProt Entry: P16104
Application Information

Molecular Weight: Predicted MW:15 kDa Observed MW:15 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB2500

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry(FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Phospho-Histone H2A.X (S139)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-phospho-Histone H2A.X (S139) antibody (Cat#71197). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-Histone H2A.X (S139) antibody (Cat#71197, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-phospho-Histone H2A.X (S139) antibody (Cat#71197). Phospho-Histone H2A.X (S139) expression in wild-type (WT) and H2AX knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-Histone H2A.X (S139) antibody (Cat#71197, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of Phospho-Histone H2A.X (S139) expression in HepG2 cells using Phospho-Histone H2A.X (S139) antibody (Cat#71197, 1：2000). Green, isotype control; red, Phospho-Histone H2A.X (S139).
  • Immunocytochemical staining of HepG2 cells with anti-Phospho-Histone H2A.X (S139) antibody (Cat#71197, 1:1000) . Nuclei were stained blue with DAPI; Phospho-Histone H2A.X (S139) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Citation(0)
  • 基因名：
    H2AX
  • 货号：
    71197
  • 应用：
    WB
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Western blotting analysis using anti-phospho-Histone H2A.X (S139) antibody (Cat#71197). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-Histone H2A.X (S139) antibody (Cat#71197, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-phospho-Histone H2A.X (S139) antibody (Cat#71197). Phospho-Histone H2A.X (S139) expression in wild-type (WT) and H2AX knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-Histone H2A.X (S139) antibody (Cat#71197, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Phospho-Histone H2A.X (S139) expression in HepG2 cells using Phospho-Histone H2A.X (S139) antibody (Cat#71197, 1：2000). Green, isotype control; red, Phospho-Histone H2A.X (S139).
Immunocytochemical staining of HepG2 cells with anti-Phospho-Histone H2A.X (S139) antibody (Cat#71197, 1:1000) . Nuclei were stained blue with DAPI; Phospho-Histone H2A.X (S139) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
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