Flow cytometric analysis of Keratin 8 expression in HepG2 cells using anti-Keratin 8 antibody (Cat#71211, 1:2,000). Green, isotype control; red, Keratin 8.
Immunocytochemical staining of HepG2 cells with anti-Keratin 8 antibody (Cat#71211, 1:1,000). Nuclei were stained blue with DAPI; Keratin 8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-keratin 8 antibody (Cat#71211). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-keratin 8 antibody (Cat#71211, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-keratin 8 antibody (Cat#71211). Keratin 8 expression in wild type (WT) and keratin 8 (KRT8) knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-keratin 8 antibody (Cat#71211, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
