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KO-Validated Anti-Fyn Rabbit Monoclonal Antibody #71213

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Western blotting analysis using anti-fyn antibody (Cat#71213). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-fyn antibody (Cat#71213, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Fyn antibody (Cat#71213). Fyn expression in wild-type (WT) and FYN knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Fyn antibody (Cat#71213, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    FYN
  • 货号:
    71213
  • 应用:
    WB
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KO-Validated Anti-Fyn Rabbit Monoclonal Antibody
Aliases FYN; FYN Proto-Oncogene, Src Family Tyrosine Kinase; SLK; SYN; FYN Oncogene Related To SRC, FGR, YES; Tyrosine-Protein Kinase Fyn; Proto-Oncogene C-Fyn; Proto-Oncogene Syn; Src-Like Kinase; EC 2.7.10.2; MGC45350; OKT3-Induced Calcium Influx Regulator; Tyrosine Kinase P59fyn(T); Src/Yes-Related Novel; C-Syn Protooncogene; EC 2.7.10; P59-FYN; P59-Fyn
Background

Gene Name: FYN

NCBI Gene Entry: 2534

UniProt Entry: P06241

Application Information

Molecular Weight: Predicted, 61 kDa; observed, 61 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB2930

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human Fyn
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-fyn antibody (Cat#71213). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-fyn antibody (Cat#71213, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-Fyn antibody (Cat#71213). Fyn expression in wild-type (WT) and FYN knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Fyn antibody (Cat#71213, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Western blotting analysis using anti-fyn antibody (Cat#71213). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-fyn antibody (Cat#71213, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Fyn antibody (Cat#71213). Fyn expression in wild-type (WT) and FYN knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Fyn antibody (Cat#71213, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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