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KO-Validated Anti-G3BP Rabbit Monoclonal Antibody #71236

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Flow cytometric analysis of G3BP expression in HepG2 cells using G3BP antibody (Cat#71236, 1:2,000). Green, isotype control; red, G3BP.
Immunocytochemical staining of HepG2 cells with G3BP antibody (Cat#71236, 1:1,000). Nuclei were stained blue with DAPI; G3BP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-G3BP antibody (Cat#71236). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-G3BP antibody (Cat#71236, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-G3BP antibody (Cat#71236). G3BP expression in wild-type (WT) and G3BP knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-G3BP antibody (Cat#71236, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    G3BP1
  • 货号：
    71236
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KO-Validated Anti-G3BP Rabbit Monoclonal Antibody
Aliases G3BP1; G3BP Stress Granule Assembly Factor 1; G3BP; HDH-VIII; Ras-GTPase-Activating Protein SH3-Domain-Binding Protein; GTPase Activating Protein (SH3 Domain) Binding Protein 1; Ras GTPase-Activating Protein-Binding Protein 1; GAP SH3 Domain-Binding Protein 1; ATP-Dependent DNA Helicase VIII; DNA Helicase VIII; G3BP-1; RasGAP-Associated Endoribonuclease G3BP; GAP Binding Protein; EC 3.6.4.12; EC 3.6.4.13; HDH VIII; EC 3.6.1
Background

Gene Name: G3BP1

NCBI Gene Entry: 10146

UniProt Entry: Q13283
Application Information

Molecular Weight: Predicted, 52 kDa; observed, 68 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB3530

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human G3BP
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of G3BP expression in HepG2 cells using G3BP antibody (Cat#71236, 1:2,000). Green, isotype control; red, G3BP.
  • Immunocytochemical staining of HepG2 cells with G3BP antibody (Cat#71236, 1:1,000). Nuclei were stained blue with DAPI; G3BP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
  • Western blotting analysis using anti-G3BP antibody (Cat#71236). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-G3BP antibody (Cat#71236, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-G3BP antibody (Cat#71236). G3BP expression in wild-type (WT) and G3BP knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-G3BP antibody (Cat#71236, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    G3BP1
  • 货号：
    71236
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of G3BP expression in HepG2 cells using G3BP antibody (Cat#71236, 1:2,000). Green, isotype control; red, G3BP.
Immunocytochemical staining of HepG2 cells with G3BP antibody (Cat#71236, 1:1,000). Nuclei were stained blue with DAPI; G3BP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-G3BP antibody (Cat#71236). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-G3BP antibody (Cat#71236, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-G3BP antibody (Cat#71236). G3BP expression in wild-type (WT) and G3BP knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-G3BP antibody (Cat#71236, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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