Flow cytometric analysis of FTO expression in HAP-1 cells using anti-FTO antibody (Cat#71240, 1:2,000). Green, isotype control; red, FTO.
Immunocytochemical staining of HAP1 cells with anti-FTO antibody (Cat#71240, 1:1,000). Nuclei were stained blue with DAPI; FTO was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-FTO antibody (Cat#71240). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FTO antibody (Cat#71240, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-FTO antibody (Cat#71240). FTO expression in wild-type (WT) and FTO knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-FTO antibody (Cat#71240, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
