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KO-Validated Anti-Catenin Beta 1 Mouse Monoclonal Antibody #71274

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Western blotting analysis using anti-catenin beta 1 antibody (Cat#71274). Catenin beta 1 expression in wild-type (WT) and catenin beta 1 (CTNNB1) knockout (KO) 293T cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-catenin beta 1 antibody (Cat#71274, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-catenin beta 1 antibody (Cat#71274). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-catenin beta 1 antibody (Cat#71274, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Catenin beta 1 expression in HAP-1 cells using anti-Catenin beta 1 antibody (Cat#71274, 1:2,000). Green, isotype control; red, Catenin beta 1.
  • 基因名:
    CTNNB1
  • 货号:
    71274
  • 应用:
    WB, FCM
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KO-Validated Anti-Catenin Beta 1 Mouse Monoclonal Antibody
Aliases CTNNB1; Catenin Beta 1; Beta-Catenin; Armadillo; CTNNB; Catenin (Cadherin-Associated Protein), Beta 1, 88kDa; Catenin Beta-1; Catenin (Cadherin-Associated Protein), Beta 1 (88kD); Catenin (Cadherin-Associated Protein), Beta 1; NEDSDV; MRD19; EVR7
Background

Gene Name: CTNNB1

NCBI Gene Entry: 1499

UniProt Entry: P35222

Application Information

Molecular Weight: Predicted, 85 kDa; observed, 85 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB5850

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human CTNNB1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1::200-1:2,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-catenin beta 1 antibody (Cat#71274). Catenin beta 1 expression in wild-type (WT) and catenin beta 1 (CTNNB1) knockout (KO) 293T cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-catenin beta 1 antibody (Cat#71274, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-catenin beta 1 antibody (Cat#71274). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-catenin beta 1 antibody (Cat#71274, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of Catenin beta 1 expression in HAP-1 cells using anti-Catenin beta 1 antibody (Cat#71274, 1:2,000). Green, isotype control; red, Catenin beta 1.
Citation(0)
Western blotting analysis using anti-catenin beta 1 antibody (Cat#71274). Catenin beta 1 expression in wild-type (WT) and catenin beta 1 (CTNNB1) knockout (KO) 293T cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-catenin beta 1 antibody (Cat#71274, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-catenin beta 1 antibody (Cat#71274). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-catenin beta 1 antibody (Cat#71274, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Catenin beta 1 expression in HAP-1 cells using anti-Catenin beta 1 antibody (Cat#71274, 1:2,000). Green, isotype control; red, Catenin beta 1.
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