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Anti-Sirtuin 6 Rabbit Monoclonal Antibody #7761

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Flow cytometric analysis of SIRT6 expression in HT-1080 cells using anti-SIRT6 antibody (Cat#7761, 1:2,000). Green, isotype control; red, SIRT6.
Immunocytochemical staining of HT-1080 cells with Sirtuin 6 antibody (Cat#7761, 1:1,000). Nuclei were stained blue with DAPI; Sirtuin 6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Sirtuin 6 antibody (Cat#7761). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Sirtuin 6 antibody (Cat#7761, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    SIRT6
  • 货号：
    7761
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-Sirtuin 6 Rabbit Monoclonal Antibody
Aliases SIRT6; Sirtuin 6; Protein Mono-ADP-Ribosyltransferase Sirtuin-6; NAD-Dependent Protein Deacetylase Sirtuin-6; NAD-Dependent Protein Deacylase Sirtuin-6; Regulatory Protein SIR2 Homolog 6; SIR2-Like Protein 6; SIR2L6; HSIRT6; Sirtuin (Silent Mating Type Information Regulation 2, S. Cerevisiae, Homolog) 6; Sirtuin (Silent Mating Type Information Regulation 2 Homolog) 6 (S. Cerevisiae); Sir2-Related Protein Type 6; Sirtuin Type 6; EC 2.3.1.286; EC 2.3.1.-; EC 2.4.2.-
Background

Gene Name: SIRT6

NCBI Gene Entry: 51548

UniProt Entry: Q8N6T7
Application Information

Molecular Weight: Predicted, 39 kDa; observed, 42 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB1135

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human SIRT6
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of SIRT6 expression in HT-1080 cells using anti-SIRT6 antibody (Cat#7761, 1:2,000). Green, isotype control; red, SIRT6.
  • Immunocytochemical staining of HT-1080 cells with Sirtuin 6 antibody (Cat#7761, 1:1,000). Nuclei were stained blue with DAPI; Sirtuin 6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-Sirtuin 6 antibody (Cat#7761). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Sirtuin 6 antibody (Cat#7761, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    SIRT6
  • 货号：
    7761
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of SIRT6 expression in HT-1080 cells using anti-SIRT6 antibody (Cat#7761, 1:2,000). Green, isotype control; red, SIRT6.
Immunocytochemical staining of HT-1080 cells with Sirtuin 6 antibody (Cat#7761, 1:1,000). Nuclei were stained blue with DAPI; Sirtuin 6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Sirtuin 6 antibody (Cat#7761). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Sirtuin 6 antibody (Cat#7761, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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