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Anti-Chromobox 4 Rabbit Monoclonal Antibody #9184

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Flow cytometric analysis of Chromobox 4 expression in HepG2 cells using Chromobox 4 antibody (Cat#9184, 1:2,000). Green, isotype control; red, Chromobox 4.

Immunocytochemical staining of HepG2 cells with Chromobox 4 antibody (Cat#9184, 1:1,000). Nuclei were stained blue with DAPI; Chromobox 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-Chromobox 4 antibody (Cat#9184). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Chromobox 4 antibody (Cat#9184, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

CBX4
货号：

9184
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-Chromobox 4 Rabbit Monoclonal Antibody
Aliases	Chromobox 4; NBP16; PC2; E3 SUMO-Protein Ligase CBX4; Chromobox Protein Homolog 4; NS5ATP1-Binding Protein 16; Polycomb 2 Homolog; HPC2; Chromobox Homolog 4 (Pc Class Homolog, Drosophila); Chromobox Homolog 4 (Drosophila Pc Class); Pc Class 2 Homolog (Drosophila); Chromobox-Like Protein 4; Chromobox Homolog 4; Pc Class 2 Homolog; EC 2.3.2.-; HPc2; Pc2
Background	Gene Name: CBX4 NCBI Gene Entry: 8535 UniProt Entry: O00257
Application Information	Molecular Weight: Predicted, 61 kDa; observed, 50 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB1905 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human CBX4
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Chromobox 4 expression in HepG2 cells using Chromobox 4 antibody (Cat#9184, 1:2,000). Green, isotype control; red, Chromobox 4.
Immunocytochemical staining of HepG2 cells with Chromobox 4 antibody (Cat#9184, 1:1,000). Nuclei were stained blue with DAPI; Chromobox 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Chromobox 4 antibody (Cat#9184). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Chromobox 4 antibody (Cat#9184, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

CBX4
货号：

9184
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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