Anti-Hypoxia inducible factor 1 subunit alpha Rabbit Monoclonal Antibody - 产品中心

Anti-Hypoxia inducible factor 1 subunit alpha Rabbit Monoclonal Antibody #9321

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Flow cytometric analysis of HIF-1-alpha expression in HT-1080 cells using anti-HIF-1-alpha antibody (Cat#9321, 1:2,000). Green, isotype control; red, HIF-1-alpha.

Immunocytochemical staining of HT-1080 cells with Hypoxia inducible factor 1 subunit alpha antibody (Cat#9321, 1:1,000) . Nuclei were stained blue with DAPI; Hypoxia inducible factor 1 subunit alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Hypoxia inducible factor 1 subunit alpha antibody (Cat#9321). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Hypoxia inducible factor 1 subunit alpha antibody (Cat#9321, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

HIF1A
货号：

9321
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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Citation(0)

Information

Product Name	Anti-Hypoxia inducible factor 1 subunit alpha Rabbit Monoclonal Antibody
Aliases	Hypoxia Inducible Factor 1 Subunit Alpha; Hypoxia Inducible Factor 1, Alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) ; Class E Basic Helix-Loop-Helix Protein 78; Basic-Helix-Loop-Helix-PAS Protein MOP1; Hypoxia-Inducible Factor 1-Alpha; PAS Domain-Containing Protein 8; Member Of PAS Protein 1; HIF-1-Alpha; BHLHe78; PASD8; MOP1; Hypoxia-Inducible Factor 1 Alpha Isoform I.3; Hypoxia Inducible Factor 1 Alpha Subunit; Hypoxia-Inducible Factor1alpha; Member Of PAS Superfamily 1; ARNT Interacting Protein; ARNT-Interacting Protein; HIF-1alpha; HIF1-ALPHA; HIF1-Alpha; BHLHE78; HIF-1A; HIF1
Background	Gene Name: HIF1A NCBI Gene Entry: 3091 UniProt Entry: Q16665
Application Information	Molecular Weight: Predicted, 93 kDa; observed, 110 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB1625 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human HIF-1-alpha
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of HIF-1-alpha expression in HT-1080 cells using anti-HIF-1-alpha antibody (Cat#9321, 1:2,000). Green, isotype control; red, HIF-1-alpha.
Immunocytochemical staining of HT-1080 cells with Hypoxia inducible factor 1 subunit alpha antibody (Cat#9321, 1:1,000) . Nuclei were stained blue with DAPI; Hypoxia inducible factor 1 subunit alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Hypoxia inducible factor 1 subunit alpha antibody (Cat#9321). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Hypoxia inducible factor 1 subunit alpha antibody (Cat#9321, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

HIF1A
货号：

9321
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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