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Anti-ALKBH1 Rabbit Monoclonal Antibody #9441

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Flow cytometric analysis of ALKBH1 expression in HepG2 cells using ALKBH1 antibody (Cat#9441, 1:2,000). Green, isotype control; red, ALKBH1.
Immunocytochemical staining of HepG2 cells with ALKBH1 antibody (Cat#9441, 1:1,000). Nuclei were stained blue with DAPI; ALKBH1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-ALKBH1 antibody (Cat#9441). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ALKBH1 antibody (Cat#9441, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    ALKBH1
  • 货号：
    9441
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-ALKBH1 Rabbit Monoclonal Antibody
Aliases AlkB Homolog 1, Histone H2A Dioxygenase; ABH; ALKBH; HABH; Alpha-Ketoglutarate-Dependent Dioxygenase ABH1; Alkylated DNA Repair Protein AlkB Homolog; DNA N6-Methyl Adenine Demethylase ALKBH1; MRNA N(3)-Methylcytidine Demethylase; DNA Oxidative Demethylase ALKBH1; Nucleic Acid Dioxygenase ALKBH1; DNA 6mA Demethylase; DNA Lyase ABH1; AlkB; ABH1; AlkB, Alkylation Repair Homolog 1 (E. Coli); AlkB, Alkylation Repair Homolog (E. Coli); TRNA N1-Methyl Adenine Demethylase; AlkB, Alkylation Repair Homolog; Alkylation Repair, AlkB Homolog; DNA Demethylase ALKBH1; EC 1.14.11.51; EC 1.14.11.33; EC 1.14.11.-; EC 4.2.99.18; ALKB
Background

Gene Name: ALKBH1

NCBI Gene Entry: 8846

UniProt Entry: Q13686
Application Information

Molecular Weight: Predicted, 44 kDa; observed, 43 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB1265

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human ALKBH1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of ALKBH1 expression in HepG2 cells using ALKBH1 antibody (Cat#9441, 1:2,000). Green, isotype control; red, ALKBH1.
  • Immunocytochemical staining of HepG2 cells with ALKBH1 antibody (Cat#9441, 1:1,000). Nuclei were stained blue with DAPI; ALKBH1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-ALKBH1 antibody (Cat#9441). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ALKBH1 antibody (Cat#9441, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    ALKBH1
  • 货号：
    9441
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of ALKBH1 expression in HepG2 cells using ALKBH1 antibody (Cat#9441, 1:2,000). Green, isotype control; red, ALKBH1.
Immunocytochemical staining of HepG2 cells with ALKBH1 antibody (Cat#9441, 1:1,000). Nuclei were stained blue with DAPI; ALKBH1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-ALKBH1 antibody (Cat#9441). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ALKBH1 antibody (Cat#9441, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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