Flow cytometric analysis of Asparagine synthetase expression in HAP-1 cells using anti-Asparagine synthetase antibody (Cat#9757, 1：2000). Green, isotype control; red, Asparagine synthetase.
Immunocytochemical staining of HAP-1 cells with anti-Asparagine synthetase antibody (Cat#9757, 1:1,000) . Nuclei were stained blue with DAPI; Asparagine synthetase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Asparagine synthetase antibody (Cat#9757). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Asparagine synthetase antibody (Cat#9757, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Asparagine synthetase expression in HAP-1 cells using anti-Asparagine synthetase antibody (Cat#9757, 1：2000). Green, isotype control; red, Asparagine synthetase.
Immunocytochemical staining of HAP-1 cells with anti-Asparagine synthetase antibody (Cat#9757, 1:1,000) . Nuclei were stained blue with DAPI; Asparagine synthetase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Asparagine synthetase antibody (Cat#9757). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Asparagine synthetase antibody (Cat#9757, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Asparagine synthetase expression in HAP-1 cells using anti-Asparagine synthetase antibody (Cat#9757, 1：2000). Green, isotype control; red, Asparagine synthetase.
Immunocytochemical staining of HAP-1 cells with anti-Asparagine synthetase antibody (Cat#9757, 1:1,000) . Nuclei were stained blue with DAPI; Asparagine synthetase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Asparagine synthetase antibody (Cat#9757). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Asparagine synthetase antibody (Cat#9757, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
