Human CHUK Knockdown Cell Line (Wb-Validated) - 产品中心

Human CHUK Knockdown Cell Line (Wb-Validated) #C1374

一键复制产品信息

返回列表

RT-qPCR analysis. HeLa cells were infected with CHUK-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.

Western blotting analysis. CHUK protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies (Cat#61374, 1:5,000) against CHUK and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

CHUK
货号：

C1374
应用：
物种反应性：

规格：

1 Kit

价格：

询价

Information

Picture

Citation(0)

Information

Product Name	Human CHUK Knockdown Cell Line (Wb-Validated)
Aliases	CHUK; Component Of Inhibitor Of Nuclear Factor Kappa B Kinase Complex; IKK-Alpha; NFKBIKA; IKK1; IKKA; Inhibitor Of Nuclear Factor Kappa-B Kinase Subunit Alpha; Conserved Helix-Loop-Helix Ubiquitous Kinase; IkBKA; TCF16; Transcription Factor 16; I-Kappa-B Kinase 1; EC 2.7.11.10; IKK-1; Nuclear Factor NF-Kappa-B Inhibitor Kinase Alpha; Nuclear Factor NFkappaB Inhibitor Kinase Alpha; IkB Kinase Alpha Subunit; I-Kappa-B Kinase-Alpha; I-Kappa-B Kinase Alpha; I-Kappa-B Kinase; IkappaB Kinase; IKK-A Kinase; EC 2.7.11; TCF-16; IKBKA; IKK-A; BPS2
Background	Gene Name: CHUK NCBI Gene Entry: 1147
Storage	Store at liquid nitrogen for 1 year.
Kit Components	1. Human CHUK Knockdown Cell Line (Wb-Validated) 2. Wild-type cell line
Parental Cell Line	Human cell line supplied by the client
Validation Methods	RT-qPCR, Western blotting (WB)
Shipping	Shipped on Dry Ice.
Instructions For Use	This knockdown cell line should be paired with wild-type cell line for use.
Note	This product is for research use only.

Picture

RT-qPCR analysis. HeLa cells were infected with CHUK-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. CHUK protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies (Cat#61374, 1:5,000) against CHUK and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

CHUK
货号：

C1374
应用：
物种反应性：

规格：

1 Kit

价格：

询价

Datasheet

首页

关于我们

善本标准

产品中心

定制服务

信号通路

促销活动

技术支持

新闻资讯

首页