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Human USP9X Knockdown Cell Line (Wb-Validated) #C61180

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RT-qPCR analysis. HeLa cells were infected with USP9X-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.

Western blotting analysis. USP9X protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against USP9X and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

基因名：

USP9X
货号：

C61180
应用：
物种反应性：

规格：

1 Kit

价格：

询价

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Product Name	Human USP9X Knockdown Cell Line (Wb-Validated)
Aliases	USP9X; Ubiquitin Specific Peptidase 9 X-Linked; DFFRX; FAF-X; MRX99; FAF; Ubiquitin Specific Protease 9, X Chromosome (Fat Facets-Like Drosophila); Probable Ubiquitin Carboxyl-Terminal Hydrolase FAF-X; Ubiquitin-Specific Protease 9, X Chromosome; Ubiquitin Carboxyl-Terminal Hydrolase 9X ; Deubiquitinating Enzyme FAF-X; Ubiquitin Thioesterase FAF-X; Fat Facets-Like, X-Linked; Fat Facets In Mammals; HFAM; FAM; Ubiquitin Specific Peptidase 9, X-Linked (Fat Facets-Like, Drosophila); Ubiquitin Specific Protease 9, X-Linked (Fat Facets-Like, Drosophila); Probable Ubiquitin Carboxyl-Terminal Hydrolase FAF-X; Ubiquitin-Specific Processing Protease FAF-X; Ubiquitin-Specific-Processing Protease FAF-X; Drosophila Fat Facets Related, X-Linked; Fat Facets Protein Related, X-Linked; Fat Facets Protein-Related, X-Linked; Ubiquitin Thiolesterase FAF-X; EC 3.4.19.12; EC 3.1.2.15; MRXS99F; XLID99; USP9
Background	Gene Name: USP9X NCBI Gene Entry: 8239
Storage	Store at liquid nitrogen for 1 year.
Kit Components	1. Human USP9X Knockdown Cell Line (Wb-Validated) 2. Wild-type cell line
Parental Cell Line	Human cell line supplied by the client
Validation Methods	RT-qPCR, Western blotting (WB)
Shipping	Shipped on Dry Ice.
Instructions For Use	This knockdown cell line should be paired with wild-type cell line for use.
Note	This product is for research use only.

Picture

RT-qPCR analysis. HeLa cells were infected with USP9X-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. USP9X protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against USP9X and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Citation(0)

基因名：

USP9X
货号：

C61180
应用：
物种反应性：

规格：

1 Kit

价格：

询价

Datasheet

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