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Human LEFTY2 Knockdown Cell Line (Wb-Validated) #C61190

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Western blotting analysis. LEFTY2 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against LEFTY2 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
RT-qPCR analysis. HT-1080 cells were infected with LEFTY2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • 基因名：
    LEFTY2
  • 货号：
    C61190
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

Picture

Citation(0)

Information
Product Name Human LEFTY2 Knockdown Cell Line (Wb-Validated)
Aliases LEFTY2; Left-Right Determination Factor 2; LEFTYA; LEFTA; TGFB4; EBAF; Endometrial Bleeding-Associated Factor; Left-Right Determination Factor A; Transforming Growth Factor Beta-4; Protein Lefty-2; Protein Lefty-A; TGF-Beta-4; Endometrial Bleeding Associated Factor (Left-Right Determination, Factor A; Transforming Growth Factor Beta Superfamily); Transforming Growth Factor, Beta-4 (Endometrial Bleeding-Associated Factor; LEFTY A)
Background

Gene Name: LEFTY2

NCBI Gene Entry: 7044
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human LEFTY2 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • Western blotting analysis. LEFTY2 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against LEFTY2 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • RT-qPCR analysis. HT-1080 cells were infected with LEFTY2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Citation(0)
  • 基因名：
    LEFTY2
  • 货号：
    C61190
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
Western blotting analysis. LEFTY2 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against LEFTY2 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
RT-qPCR analysis. HT-1080 cells were infected with LEFTY2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
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