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Human PPP3CA Knockdown Cell Line (Wb-Validated) #C61275

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RT-qPCR analysis. HeLa cells were infected with PPP3CA-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PPP3CA protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies (Cat#61275, 1:5,000) against PPP3CA and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    PPP3CA
  • 货号:
    C61275
  • 应用:
  • 物种反应性:
规格:
1 Kit
价格:
询价
Information
Product Name Human PPP3CA Knockdown Cell Line (Wb-Validated)
Aliases PPP3CA; Protein Phosphatase 3 Catalytic Subunit Alpha; Calcineurin A Alpha; PPP2B; CALNA; CNA1; CAM-PRP Catalytic Subunit; EC 3.1.3.16; CNA Alpha; CALN; Protein Phosphatase 3 (Formerly 2B), Catalytic Subunit, Alpha Isoform (Calcineurin A Alpha); Serine/Threonine-Protein Phosphatase 2B Catalytic Subunit Alpha Isoform; Protein Phosphatase 3 (Formerly 2B), Catalytic Subunit, Alpha Isoform; Protein Phosphatase 2B, Catalytic Subunit, Alpha Isoform; Calmodulin-Dependent Calcineurin A Subunit Alpha Isoform; Protein Phosphatase 3, Catalytic Subunit, Alpha Isozyme; Protein Phosphatase 3 Catalytic Subunit Alpha Isozyme; ACCIID; CALNA1; IECEE1; DEE91; IECEE; CCN1; CNA
Background

Gene Name: PPP3CA

NCBI Gene Entry: 5530

Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human PPP3CA Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with PPP3CA-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. PPP3CA protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies (Cat#61275, 1:5,000) against PPP3CA and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
RT-qPCR analysis. HeLa cells were infected with PPP3CA-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PPP3CA protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies (Cat#61275, 1:5,000) against PPP3CA and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
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