• 一抗
  • 二抗
  • 慢病毒
  • 细胞系
  • 裂解物
  • 试剂类

Human ALAS1 Knockdown Cell Line (Wb-Validated) #C61368

一键复制产品信息
RT-qPCR analysis. HeLa cells were infected with ALAS1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. ALAS1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against ALAS1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名:
    ALAS1
  • 货号:
    C61368
  • 应用:
  • 物种反应性:
规格:
1 Kit
价格:
询价
Information
Product Name Human ALAS1 Knockdown Cell Line (Wb-Validated)
Aliases ALAS1; 5'-Aminolevulinate Synthase 1; ALAS-H; ALAS3; 5-Aminolevulinate Synthase, Non-Specific, Mitochondrial; Aminolevulinate, Delta-, Synthase 1; 5-Aminolevulinic Acid Synthase 1; Delta-Aminolevulinate Synthase 1; Delta-ALA Synthase 1; EC 2.3.1.37; ALASH; ALAS; 5-Aminolevulinate Synthase, Nonspecific, Mitochondrial; MigRation-Inducing Protein 4; EC 2.3.1; MIG4
Background

Gene Name: ALAS1

NCBI Gene Entry: 211

Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human ALAS1 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with ALAS1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. ALAS1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against ALAS1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
RT-qPCR analysis. HeLa cells were infected with ALAS1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. ALAS1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against ALAS1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 联系电话

    0551-63635518

    0551-65578998

  • 联系邮箱

    support@genuinbiotech.cn

    sales@genuinbiotech.cn

微信公众号

Copyright © 2025 合肥善本生物科技有限公司

All Rights Reserved. 皖ICP备2023000814号