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Human FKBP5 Knockdown Cell Line (Wb-Validated) #C61403

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RT-qPCR analysis. HeLa cells were infected with FKBP5-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis.FKBP5 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies against FKBP5 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using NaQ™ ECL Substrate Kit.
  • 基因名：
    FKBP5
  • 货号：
    C61403
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

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Information
Product Name Human FKBP5 Knockdown Cell Line (Wb-Validated)
Aliases FKBP5; FKBP Prolyl Isomerase 5; FKBP51; FKBP54; P54; 54 KDa Progesterone Receptor-Associated Immunophilin; Peptidyl-Prolyl Cis-Trans Isomerase FKBP5; 51 KDa FK506-Binding Protein; Androgen-Regulated Protein 6; HSP90-Binding Immunophilin; FK506-Binding Protein 5; FK506 Binding Protein 5; PPIase FKBP5; 51 KDa FKBP; FF1 Antigen; EC 5.2.1.8; Rotamase; FKBP-51; PPIase; Ptg-10; AIG6; Peptidylprolyl Cis-Trans Isomerase; T-Cell FK506-Binding Protein; PPIASE; PTG-10; FKBP-5
Background

Gene Name: FKBP5

NCBI Gene Entry: 2289
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human FKBP5 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with FKBP5-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis.FKBP5 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies against FKBP5 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using NaQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    FKBP5
  • 货号：
    C61403
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with FKBP5-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis.FKBP5 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies against FKBP5 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using NaQ™ ECL Substrate Kit.
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