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Human HSPA5 Knockdown Cell Line (Wb-Validated) #C61474

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RT-qPCR analysis. 293T cells were infected with HSPA5-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. HSPA5 protein expression in wild-type (WT) and shRNA knockdown (KD) 293T cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against HSPA5 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    HSPA5
  • 货号：
    C61474
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

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Information
Product Name Human HSPA5 Knockdown Cell Line (Wb-Validated)
Aliases HSPA5; Heat Shock Protein Family A (Hsp70) Member 5; GRP78; Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa); Immunoglobulin Heavy Chain-Binding Protein; Heat Shock Protein 70 Family Protein 5; Heat Shock Protein Family A Member 5; Endoplasmic Reticulum Chaperone BiP; Glucose-Regulated Protein, 78kDa; 78 KDa Glucose-Regulated Protein; Binding-Immunoglobulin Protein; HSP70 Family Protein 5; BiP; BIP; Heat Shock 70kD Protein 5 (Glucose-Regulated Protein, 78kD); Endoplasmic Reticulum Lumenal Ca(2+)-Binding Protein Grp78; Epididymis Secretory Sperm Binding Protein Li 89n; EC 3.6.4.10; HEL-S-89n; GRP-78
Background

Gene Name: HSPA5

NCBI Gene Entry: 3309
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human HSPA5 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. 293T cells were infected with HSPA5-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. HSPA5 protein expression in wild-type (WT) and shRNA knockdown (KD) 293T cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against HSPA5 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    HSPA5
  • 货号：
    C61474
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. 293T cells were infected with HSPA5-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. HSPA5 protein expression in wild-type (WT) and shRNA knockdown (KD) 293T cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against HSPA5 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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