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Human PAFAH1B1 Knockdown Cell Line (Wb-Validated) #C61494

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RT-qPCR analysis. HeLa cells were infected with PAFAH1B1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PAFAH1B1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies (Cat#61494, 1:5,000) against PAFAH1B1 and Hsp90 α , respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    PAFAH1B1
  • 货号：
    C61494
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

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Information
Product Name Human PAFAH1B1 Knockdown Cell Line (Wb-Validated)
Aliases PAFAH1B1; Platelet Activating Factor Acetylhydrolase 1b Regulatory Subunit 1; LIS1; PAFAH; MDCR; MDS; Platelet-Activating Factor Acetylhydrolase 1b, Regulatory Subunit 1 (45kDa); Platelet-Activating Factor Acetylhydrolase IB Subunit Beta; NudF; Platelet-Activating Factor Acetylhydrolase, Isoform Ib, Alpha Subunit (45kD); Platelet-Activating Factor Acetylhydrolase, Isoform Ib, Alpha Subunit 45kDa; Platelet-Activating Factor Acetylhydrolase, Isoform Ib, Subunit 1 (45kDa); Miller-Dieker Syndrome Chromosome Region; PAF Acetylhydrolase 45 KDa Subunit; Lissencephaly 1 Protein; Lissencephaly-1 Protein; PAF-AH 45 KDa Subunit; Lissencephaly-1; PAF-AH Alpha; PAFAH Alpha; PAFAHA; LIS-1; LIS2; NUDF
Background

Gene Name: PAFAH1B1

NCBI Gene Entry: 5048
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human PAFAH1B1 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with PAFAH1B1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. PAFAH1B1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies (Cat#61494, 1:5,000) against PAFAH1B1 and Hsp90 α , respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    PAFAH1B1
  • 货号：
    C61494
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with PAFAH1B1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PAFAH1B1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies (Cat#61494, 1:5,000) against PAFAH1B1 and Hsp90 α , respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
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