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Human PPM1G Knockdown Cell Line (Wb-Validated) #C61501

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RT-qPCR analysis. HeLa cells were infected with PPM1G-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PPM1G protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  β-Tubulin served as a loading control. The blots were incubated with primary antibodies (Cat#61500, 1:5,000) against PPM1G and β-Tubulin , respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    PPM1G
  • 货号：
    C61501
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

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Information
Product Name Human PPM1G Knockdown Cell Line (Wb-Validated)
Aliases PPM1G; Protein Phosphatase, Mg2+/Mn2+ Dependent 1G; PP2CG; Protein Phosphatase Magnesium-Dependent 1 Gamma; Protein Phosphatase 1G ; Protein Phosphatase 1C; EC 3.1.3.16; PP2C-Gamma; PP2CGAMMA; Protein Phosphatase 1G (Formerly 2C), Magnesium-Dependent, Gamma Isoform; Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G; Protein Phosphatase 2C, Gamma Isoform; Protein Phosphatase 2C Isoform Gamma; PP2C, Gamma; PP2Cgamma; PPP2CG; PPM1C
Background

Gene Name: PPM1G

NCBI Gene Entry: 5496
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human PPM1G Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with PPM1G-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. PPM1G protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  β-Tubulin served as a loading control. The blots were incubated with primary antibodies (Cat#61500, 1:5,000) against PPM1G and β-Tubulin , respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    PPM1G
  • 货号：
    C61501
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with PPM1G-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PPM1G protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  β-Tubulin served as a loading control. The blots were incubated with primary antibodies (Cat#61500, 1:5,000) against PPM1G and β-Tubulin , respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
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