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Human PRKAA1 Knockdown Cell Line (Wb-Validated) #C61503

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RT-qPCR analysis. HeLa cells were infected with PRKAA1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.

Western blotting analysis. PRKAA1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies (Cat#61502, 1:5,000) against PRKAA1 and β-Tubulin , respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

PRKAA1
货号：

C61503
应用：
物种反应性：

规格：

1 Kit

价格：

询价

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Product Name	Human PRKAA1 Knockdown Cell Line (Wb-Validated)
Aliases	PRKAA1; Protein Kinase AMP-Activated Catalytic Subunit Alpha 1; 5'-AMP-Activated Protein Kinase Catalytic Subunit Alpha-1; Protein Kinase, AMP-Activated, Alpha 1 Catalytic Subunit; Hydroxymethylglutaryl-CoA Reductase Kinase; Acetyl-CoA Carboxylase Kinase; Tau-Protein Kinase PRKAA1; AMPK Subunit Alpha-1; AMPK, Alpha, 1; ACACA Kinase; HMGCR Kinase; EC 2.7.11.1; AMPKa1; 5'-AMP-Activated Protein Kinase, Catalytic Alpha-1 Chain; AMP-Activated Protein Kinase, Catalytic, Alpha-1; AMP -Activate Kinase Alpha 1 Subunit; AMPK Alpha 1; EC 2.7.11.27; EC 2.7.11.31; EC 2.7.11.26; EC 2.7.11; AMPKA1; AMPK1; AMPK
Background	Gene Name: PRKAA1 NCBI Gene Entry: 5562
Storage	Store at liquid nitrogen for 1 year.
Kit Components	1. Human PRKAA1 Knockdown Cell Line (Wb-Validated) 2. Wild-type cell line
Parental Cell Line	Human cell line supplied by the client
Validation Methods	RT-qPCR, Western blotting (WB)
Shipping	Shipped on Dry Ice.
Instructions For Use	This knockdown cell line should be paired with wild-type cell line for use.
Note	This product is for research use only.

Picture

RT-qPCR analysis. HeLa cells were infected with PRKAA1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PRKAA1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies (Cat#61502, 1:5,000) against PRKAA1 and β-Tubulin , respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

PRKAA1
货号：

C61503
应用：
物种反应性：

规格：

1 Kit

价格：

询价

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