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Human AP1G1 Knockdown Cell Line (Wb-Validated) #C61609

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RT-qPCR analysis. HeLa cells were infected with AP1G1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. AP1G1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against AP1G1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    AP1G1
  • 货号：
    C61609
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

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Information
Product Name Human AP1G1 Knockdown Cell Line (Wb-Validated)
Aliases AP1G1; Adaptor Related Protein Complex 1 Subunit Gamma 1; Gamma1-Adaptin; CLAPG1; ADTG; Clathrin Assembly Protein Complex 1 Gamma-1 Large Chain; Adaptor Related Protein Complex 1 Gamma 1 Subunit; Golgi Adaptor HA1/AP1 Adaptin Subunit Gamma-1; Adaptor Protein Complex AP-1 Subunit Gamma-1; AP-1 Complex Subunit Gamma-1; Clathrin-Associated/Assembly/Adaptor Protein, Large, Gamma 1; Clathrin Assembly Protein Complex 1 Gamma Large Chain; Adaptor-Related Protein Complex 1, Gamma 1 Subunit; Adapter-Related Protein Complex 1 Subunit Gamma-1; Adaptor-Related Protein Complex 1 Subunit Gamma-1; Golgi Adaptor HA1/AP1 Adaptin Gamma Subunit; Testicular Tissue Protein Li 21; Gamma Adaptin; USRISD
Background

Gene Name: AP1G1

NCBI Gene Entry: 164
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human AP1G1 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with AP1G1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. AP1G1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against AP1G1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    AP1G1
  • 货号：
    C61609
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with AP1G1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. AP1G1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against AP1G1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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