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Human BACE1 Knockdown Cell Line (Wb-Validated) #C61888

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RT-qPCR analysis. HT-1080 cells were infected with BACE1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. BACE1 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against BACE1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    BACE1
  • 货号：
    C61888
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

Picture

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Information
Product Name Human BACE1 Knockdown Cell Line (Wb-Validated)
Aliases BACE1; Beta-Secretase 1; BACE; Membrane-Associated Aspartic Protease; Beta-Site APP Cleaving Enzyme 1; Beta-Site APP-Cleaving Enzyme
Aspartyl Protease; EC 3.4.23.46; Memapsin-2; Asp 2; ASP2; Beta-Site Amyloid Beta A4 Precursor Protein-Cleaving Enzyme; Beta-Site Amyloid Precursor Protein Cleaving Enzyme 1; Transmembrane Aspartic Proteinase Asp2; Beta-Site APP-Cleaving Enzyme 1; APP Beta-Secretase; EC 3.4.23; KIAA1149; HSPC104
Background

Gene Name: BACE1

NCBI Gene Entry: 23621
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human BACE1 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HT-1080 cells were infected with BACE1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. BACE1 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against BACE1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    BACE1
  • 货号：
    C61888
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HT-1080 cells were infected with BACE1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. BACE1 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against BACE1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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