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Human GCLM Knockdown Cell Line (Wb-Validated) #C62007

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RT-qPCR analysis. HeLa cells were infected with GCLM-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.

Western blotting analysis. GCLM protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies (Cat#62007, 1:5,000) against GCLM and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

GCLM
货号：

C62007
应用：
物种反应性：

规格：

1 Kit

价格：

询价

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Product Name	Human GCLM Knockdown Cell Line (Wb-Validated)
Aliases	GCLM; Glutamate-Cysteine Ligase Modifier Subunit; GLCLR; Gamma-Glutamylcysteine Synthetase Regulatory Subunit; Glutamate--Cysteine Ligase Regulatory Subunit; Gamma-ECS Regulatory Subunit; GCS Light Chain; Glutamate-Cysteine Ligase (Gamma-Glutamylcysteine Synthetase), Regulatory (30.8kD); Glutamate-Cysteine Ligase Modifier Subunit Delta2 Alternative Splicing; Glutamate-Cysteine Ligase Regulatory Protein; Glutamate-Cysteine Ligase, Modifier Subunit; Glutamate--Cysteine Ligase Modifier Subunit; Gamma-Glutamylcysteine Synthetase; GSC Light Chain
Background	Gene Name: GCLM NCBI Gene Entry: 2730
Storage	Store at liquid nitrogen for 1 year.
Kit Components	1. Human GCLM Knockdown Cell Line (Wb-Validated) 2. Wild-type cell line
Parental Cell Line	Human cell line supplied by the client
Validation Methods	RT-qPCR, Western blotting (WB)
Shipping	Shipped on Dry Ice.
Instructions For Use	This knockdown cell line should be paired with wild-type cell line for use.
Note	This product is for research use only.

Picture

RT-qPCR analysis. HeLa cells were infected with GCLM-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. GCLM protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies (Cat#62007, 1:5,000) against GCLM and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

GCLM
货号：

C62007
应用：
物种反应性：

规格：

1 Kit

价格：

询价

Datasheet

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