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Human PCBD1 Knockdown Cell Line (Wb-Validated) #C62427

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RT-qPCR analysis. HeLa cells were infected with PCBD1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.

Western blotting analysis. PCBD1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against PCBD1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

基因名：

PCBD1
货号：

C62427
应用：
物种反应性：

规格：

1 Kit

价格：

询价

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Product Name	Human PCBD1 Knockdown Cell Line (Wb-Validated)
Aliases	PCBD1; Pterin-4 Alpha-Carbinolamine DehydRatase 1; PCD; DCOH; PCBD; 6-Pyruvoyl-Tetrahydropterin Synthase/Dimerization Cofactor Of Hepatocyte Nuclear Factor 1 Alpha (TCF1); Pterin-4 Alpha-Carbinolamine DehydRatase/Dimerization Cofactor Of Hepatocyte Nuclear Factor 1 Alpha; Phenylalanine Hydroxylase-Stimulating Protein; 4-Alpha-Hydroxy-Tetrahydropterin DehydRatase; Pterin-4-Alpha-Carbinolamine DehydRatase; Dimerizing Cofactor For HNF1; EC 4.2.1.96; PHS; Pterin-4 Alpha-Carbinolamine DehydRatase/Dimerization Cofactor Of Hepatocyte Nuclear Factor 1 Alpha (TCF1); Pterin-4a-Carbinolamine DehydRatase (Dimerization Cofactor Of Hepatic Nuclear Factor 1-Alpha); Dimerization Cofactor Of Hepatocyte Nuclear Factor 1-Alpha; Pterin-4-Alpha Carbinolamine DehydRatase; Pterin Carbinolamine DehydRatase; Dimerization Cofactor Of HNF1; DCoH
Background	Gene Name: PCBD1 NCBI Gene Entry: 5092
Storage	Store at liquid nitrogen for 1 year.
Kit Components	1. Human PCBD1 Knockdown Cell Line (Wb-Validated) 2. Wild-type cell line
Parental Cell Line	Human cell line supplied by the client
Validation Methods	RT-qPCR, Western blotting (WB)
Shipping	Shipped on Dry Ice.
Instructions For Use	This knockdown cell line should be paired with wild-type cell line for use.
Note	This product is for research use only.

Picture

RT-qPCR analysis. HeLa cells were infected with PCBD1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PCBD1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against PCBD1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Citation(0)

基因名：

PCBD1
货号：

C62427
应用：
物种反应性：

规格：

1 Kit

价格：

询价

Datasheet

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