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Human RHOA Knockdown Cell Line (Wb-Validated) #C62443

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RT-qPCR analysis. HT-1080 cells were infected with RHOA-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. RHOA protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against RHOA and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    RHOA
  • 货号：
    C62443
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

Picture

Citation(0)

Information
Product Name Human RHOA Knockdown Cell Line (Wb-Validated)
Aliases Ras Homolog Family Member A; RHOH12; Rho12; ARH12; ARHA; Transforming Protein RhoA; Epididymis Secretory Sperm Binding Protein; Ras Homolog Gene Family, Member A; Aplysia Ras-Related Homolog 12; Small GTP Binding Protein RhoA; Rho CDNA Clone 12; Oncogene RHO H12; EC 3.6.5.2; EDFAOB; RHO12; H12
Background

Gene Name: RHOA

NCBI Gene Entry: 387
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human RHOA Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HT-1080 cells were infected with RHOA-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. RHOA protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against RHOA and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    RHOA
  • 货号：
    C62443
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HT-1080 cells were infected with RHOA-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. RHOA protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against RHOA and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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