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Human PTBP1 Knockdown Cell Line (Wb-Validated) #C62491

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RT-qPCR analysis. HeLa cells were infected with PTBP1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.

Western blotting analysis. PTBP1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against PTBP1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

基因名：

PTBP1
货号：

C62491
应用：
物种反应性：

规格：

1 Kit

价格：

询价

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Product Name	Human PTBP1 Knockdown Cell Line (Wb-Validated)
Aliases	PTBP1; Polypyrimidine Tract Binding Protein 1; Heterogeneous Nuclear Ribonucleoprotein I; HNRNP-I; HNRPI; PTB-1; PTB2; PTB3; PTB4; PPTB; PTB; Polypyrimidine Tract Binding Protein (Heterogeneous Nuclear Ribonucleoprotein I); Polypyrimidine Tract-Binding Protein 1; 57 KDa RNA-Binding Protein PPTB-1; HnRNP I; Heterogeneous Nuclear Ribonucleoprotein Polypeptide I; RNA-Binding Protein; HNRNPI; PTB-T
Background	Gene Name: PTBP1 NCBI Gene Entry: 5725
Storage	Store at liquid nitrogen for 1 year.
Kit Components	1. Human PTBP1 Knockdown Cell Line (Wb-Validated) 2. Wild-type cell line
Parental Cell Line	Human cell line supplied by the client
Validation Methods	RT-qPCR, Western blotting (WB)
Shipping	Shipped on Dry Ice.
Instructions For Use	This knockdown cell line should be paired with wild-type cell line for use.
Note	This product is for research use only.

Picture

RT-qPCR analysis. HeLa cells were infected with PTBP1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PTBP1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against PTBP1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Citation(0)

基因名：

PTBP1
货号：

C62491
应用：
物种反应性：

规格：

1 Kit

价格：

询价

Datasheet

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