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Human SFPQ Knockdown Cell Line (Wb-Validated) #C62518

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RT-qPCR analysis. HeLa cells were infected with SFPQ-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.

Western blotting analysis. SFPQ protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against SFPQ and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

基因名：

SFPQ
货号：

C62518
应用：
物种反应性：

规格：

1 Kit

价格：

询价

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Product Name	Human SFPQ Knockdown Cell Line (Wb-Validated)
Aliases	SFPQ; Splicing Factor Proline And Glutamine Rich; PSF; PPP1R140; Polypyrimidine Tract Binding Protein Associated; Protein Phosphatase 1, Regulatory Subunit 140; DNA-Binding P52/P100 Complex, 100 KDa Subunit; Splicing Factor, Proline- And Glutamine-Rich; Splicing Factor Proline/Glutamine-Rich; 100 KDa DNA-Pairing Protein; Splicing Factor Proline/Glutamine Rich (Polypyrimidine Tract-Binding Protein-Associated); Splicing Factor Proline/Glutamine Rich (Polypyrimidine Tract Binding Protein Associated); Polypyrimidine Tract-Binding Protein-Associated Splicing Factor; Polypyrimidine Tract-Binding Protein-Associated-Splicing Factor; Epididymis Secretory Sperm Binding Protein; PTB-Associated Splicing Factor; PTB-Associated-Splicing Factor; HPOMp100; POMP100
Background	Gene Name: SFPQ NCBI Gene Entry: 6421
Storage	Store at liquid nitrogen for 1 year.
Kit Components	1. Human SFPQ Knockdown Cell Line (Wb-Validated) 2. Wild-type cell line
Parental Cell Line	Human cell line supplied by the client
Validation Methods	RT-qPCR, Western blotting (WB)
Shipping	Shipped on Dry Ice.
Instructions For Use	This knockdown cell line should be paired with wild-type cell line for use.
Note	This product is for research use only.

Picture

RT-qPCR analysis. HeLa cells were infected with SFPQ-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. SFPQ protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against SFPQ and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Citation(0)

基因名：

SFPQ
货号：

C62518
应用：
物种反应性：

规格：

1 Kit

价格：

询价

Datasheet

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