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Human PRKCSH Knockdown Cell Line (Wb-Validated) #C62541

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RT-qPCR analysis. HeLa cells were infected with PRKCSH-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PRKCSH protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against PRKCSH and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    PRKCSH
  • 货号：
    C62541
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

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Information
Product Name Human PRKCSH Knockdown Cell Line (Wb-Validated)
Aliases PRKCSH; Protein Kinase C Substrate 80K-H; Glucosidase 2 Subunit Beta; VASAP-60; PKCSH; G19P1; GIIB; Protein Kinase C Substrate 60.1 KDa Protein Heavy Chain; Advanced Glycation End-Product Receptor 2; Glucosidase II Subunit Beta; Hepatocystin; GluIIbeta; GIIbeta; AGE-R2; 80K-H; PCLD; PLD1; Protein Kinase C Substrate, 80 Kda Protein; Glucosidase II Beta Subuni; Polycystic Liver Disease; AGE-Binding Receptor 2; 80K-H Protein; PCLD1
Background

Gene Name: PRKCSH

NCBI Gene Entry: 5589
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human PRKCSH Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with PRKCSH-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. PRKCSH protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against PRKCSH and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    PRKCSH
  • 货号：
    C62541
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with PRKCSH-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PRKCSH protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against PRKCSH and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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