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Human SCARB2 Knockdown Cell Line (Wb-Validated) #C62586

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RT-qPCR analysis. HeLa cells were infected with SCARB2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. SCARB2 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against SCARB2 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    SCARB2
  • 货号：
    C62586
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

Picture

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Information
Product Name Human SCARB2 Knockdown Cell Line (Wb-Validated)
Aliases Scavenger Receptor Class B Member 2; LIMPII; Lysosome Membrane Protein 2; HLGP85; SR-BII; LIMP-2; CD36L2; CD36 Antigen (Collagen Type I Receptor, Thrombospondin Receptor)-Like 2 (Lysosomal Integral Membrane Protein II); 85 KDa Lysosomal Membrane Sialoglycoprotein; Lysosome Membrane Protein II; CD36 Antigen-Like 2; LIMP II; LGP85; 85 KDa Lysosomal Sialoglycoprotein Scavenger Receptor Class B, Member 2;Lysosomal Integral Membrane Protein II; CD36 Antigen; LIMP2; AMRF; EPM4
Background

Gene Name: SCARB2

NCBI Gene Entry: 950
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human SCARB2 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with SCARB2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. SCARB2 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against SCARB2 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    SCARB2
  • 货号：
    C62586
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with SCARB2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. SCARB2 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against SCARB2 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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