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Human SMARCA4 Knockdown Cell Line (Wb-Validated) #C62628

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RT-qPCR analysis. HeLa cells were infected with SMARCA4-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.

Western blotting analysis.SMARCA4 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against SMARCA4 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

基因名：

SMARCA4
货号：

C62628
应用：
物种反应性：

规格：

1 Kit

价格：

询价

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Product Name	Human SMARCA4 Knockdown Cell Line (Wb-Validated)
Aliases	SMARCA4; SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily A, Member 4; BRG1; Mitotic Growth And Transcription Activator; ATP-Dependent Helicase SMARCA4; SNF2-BETA; HSNF2b; BAF190; SNF2LB; SNF2L4; SNF2; SWI2; Global Transcription Activator Homologous Sequence; Sucrose Nonfermenting-Like 4; Transcription Activator BRG1; BRG1-Associated Factor 190A; Protein Brahma Homolog 1; BRM/SWI2-Related Gene 1; Homeotic Gene Regulator; Brahma Protein-Like 1; Nuclear Protein GRB1; Protein BRG-1; SNF2-Like 4; FLJ39786; BAF190A; SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Member 4; EC 3.6.4.-; SNF2-Beta; EC 3.6.1; MRD16; RTPS2; SNF2B; CSS4
Background	Gene Name: SMARCA4 NCBI Gene Entry: 6597
Storage	Store at liquid nitrogen for 1 year.
Kit Components	1. Human SMARCA4 Knockdown Cell Line (Wb-Validated) 2. Wild-type cell line
Parental Cell Line	Human cell line supplied by the client
Validation Methods	RT-qPCR, Western blotting (WB)
Shipping	Shipped on Dry Ice.
Instructions For Use	This knockdown cell line should be paired with wild-type cell line for use.
Note	This product is for research use only.

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RT-qPCR analysis. HeLa cells were infected with SMARCA4-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis.SMARCA4 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against SMARCA4 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Citation(0)

基因名：

SMARCA4
货号：

C62628
应用：
物种反应性：

规格：

1 Kit

价格：

询价

Datasheet

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