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Human SMC4 Knockdown Cell Line (Wb-Validated) #C62727

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RT-qPCR analysis. HeLa cells were infected with SMC4-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. SMC4 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against SMC4 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名:
    SMC4
  • 货号:
    C62727
  • 应用:
  • 物种反应性:
规格:
1 Kit
价格:
询价
Information
Product Name Human SMC4 Knockdown Cell Line (Wb-Validated)
Aliases SMC4; Structural Maintenance Of Chromosomes 4; HCAP-C; SMC4L1; CAP-C; Structural Maintenance Of Chromosomes Protein 4; Chromosome-Associated Polypeptide C; SMC Protein 4; SMC-4; CAPC; SMC4 (Structural Maintenance Of Chromosomes 4, Yeast)-Like 1; SMC4 Structural Maintenance Of Chromosomes 4-Like 1 (Yeast); SMC4 Structural Maintenance Of Chromosomes 4-Like 1; XCAP-C Homolog
Background

Gene Name: SMC4

NCBI Gene Entry: 10051

Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human SMC4 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with SMC4-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. SMC4 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against SMC4 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
RT-qPCR analysis. HeLa cells were infected with SMC4-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. SMC4 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against SMC4 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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