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Human LRP1 Knockdown Cell Line (Wb-Validated) #C63298

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RT-qPCR analysis. HeLa cells were infected with LRP1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. LRP1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against LRP1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    LRP1
  • 货号：
    C63298
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

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Information
Product Name Human LRP1 Knockdown Cell Line (Wb-Validated)
Aliases LRP1; LDL Receptor Related Protein 1; APOER; Alpha-2-Macroglobulin Receptor; IGFBP3R1; IGFBP-3R; LRP1A; CD91;A2MR（原A2Human）; LRP; APR; Prolow-Density Lipoprotein Receptor-Related Protein 1; Low Density Lipoprotein Receptor-Related Protein 1; Apolipoprotein E Receptor; Transforming Growth Factor-β Receptor Type V; TbetaR-V/LRP-1/IGFBP-3 Receptor; Type V Tgf-Beta Receptor; CD91 Antigen; EC 3.4.21.9; EC 1.1.1.94; IGFBP3R; TGFBR5; LRP-1; DDH3; KPA
Background

Gene Name: LRP1

NCBI Gene Entry: 4035
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human LRP1 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with LRP1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. LRP1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against LRP1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    LRP1
  • 货号：
    C63298
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with LRP1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. LRP1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against LRP1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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