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Human AP3M1 Knockdown Cell Line (Wb-Validated) #C63402

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RT-qPCR analysis. HeLa cells were infected with AP3M1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. AP3M1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against AP3M1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名:
    AP3M1
  • 货号:
    C63402
  • 应用:
  • 物种反应性:
规格:
1 Kit
价格:
询价
Information
Product Name Human AP3M1 Knockdown Cell Line (Wb-Validated)
Aliases AP3M1; Adaptor Related Protein Complex 3 Subunit Mu 1; Adaptor Related Protein Complex 3 Mu 1 Subunit; AP-3 Complex Subunit Mu-1; Mu-Adaptin 3A; Mu3A-Adaptin; Adapter-Related Protein Complex 3 Mu-1 Subunit; Adaptor-Related Protein Complex 3 Subunit Mu-1; Clathrin Adaptor Complex AP3, Mu-3A Subunit; AP-3 Adapter Complex Mu3A Subunit; AP-3 Adaptor Complex Mu3A Subunit
Background

Gene Name: AP3M1

NCBI Gene Entry: 26985

Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human AP3M1 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with AP3M1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. AP3M1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against AP3M1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
RT-qPCR analysis. HeLa cells were infected with AP3M1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. AP3M1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against AP3M1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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