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Human AKR1A1 Knockdown Cell Line (Wb-Validated) #C63498

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RT-qPCR analysis. HeLa cells were infected with AKR1A1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. AKR1A1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against AKR1A1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    AKR1A1
  • 货号：
    C63498
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

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Information
Product Name Human AKR1A1 Knockdown Cell Line (Wb-Validated)
Aliases AKR1A1; Aldo-Keto Reductase Family 1 Member A1; ALR; Aldehyde Reductase; DD3; Dihydrodiol Dehydrogenase 3; Glucuronolactone Reductase; Glucuronate Reductase; EC 1.1.1.2; ALDR1; Aldo-Keto Reductase Family 1, Member A1 (Aldehyde Reductase); Epididymis Secretory Sperm Binding Protein Li 165mP; Epididymis Secretory Protein Li 6; Alcohol Dehydrogenase [NADP(+)]; Alcohol Dehydrogenase; EC 1.1.1.372; HEL-S-165mP; EC 1.1.1.54; EC 1.1.1.19; EC 1.1.1.20; EC 1.1.1; HEL-S-6; ARM; ScorR; EC 1.6.-.-
Background

Gene Name: AKR1A1

NCBI Gene Entry: 10327
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human AKR1A1 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with AKR1A1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. AKR1A1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against AKR1A1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    AKR1A1
  • 货号：
    C63498
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with AKR1A1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. AKR1A1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against AKR1A1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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