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Human HADHA Knockdown Cell Line (Wb-Validated) #C63587

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RT-qPCR analysis. HeLa cells were infected with HADHA-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.

Western blotting analysis. HADHA protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against HADHA and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

基因名：

HADHA
货号：

C63587
应用：
物种反应性：

规格：

1 Kit

价格：

询价

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Product Name	Human HADHA Knockdown Cell Line (Wb-Validated)
Aliases	HADHA; Hydroxyacyl-CoA Dehydrogenase Trifunctional Multienzyme Complex Subunit Alpha; LCHAD; LCEH; MTPA; GBP; Hydroxyacyl-Coenzyme A Dehydrogenase/3-Ketoacyl-Coenzyme A Thiolase/Enoyl-Coenzyme A HydRatase (Trifunctional Protein), Alpha Subunit; Hydroxyacyl-CoA Dehydrogenase/3-Ketoacyl-CoA Thiolase/Enoyl-CoA HydRatase (Trifunctional Protein), Alpha Subunit; Mitochondrial Trifunctional Protein, Alpha Subunit; Trifunctional Enzyme Subunit Alpha, Mitochondrial; Long-Chain-3-Hydroxyacyl-CoA Dehydrogenase; Monolysocardiolipin Acyltransferase; Long-Chain 2-Enoyl-CoA HydRatase; 78 KDa Gastrin-Binding Protein; Gastrin-Binding Protein; HADH; Mitochondrial Long-Chain L-3-Hydroxyacyl-Coenzyme A (CoA) Dehydrogenase, Alpha Subunit; Mitochondrial Long-Chain 2-Enoyl-Coenzyme A (CoA) HydRatase, Alpha Subunit; 3-Ketoacyl-Coenzyme A (CoA) Thiolase, Alpha Subunit; Mitochondrial Trifunctional Enzyme, Alpha Subunit; 3-Oxoacyl-CoA Thiolase; EC 2.3.1.- ; TP-ALPHA; TP-Alpha; ECHA
Background	Gene Name: HADHA NCBI Gene Entry: 3030
Storage	Store at liquid nitrogen for 1 year.
Kit Components	1. Human HADHA Knockdown Cell Line (Wb-Validated) 2. Wild-type cell line
Parental Cell Line	Human cell line supplied by the client
Validation Methods	RT-qPCR, Western blotting (WB)
Shipping	Shipped on Dry Ice.
Instructions For Use	This knockdown cell line should be paired with wild-type cell line for use.
Note	This product is for research use only.

Picture

RT-qPCR analysis. HeLa cells were infected with HADHA-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. HADHA protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against HADHA and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Citation(0)

基因名：

HADHA
货号：

C63587
应用：
物种反应性：

规格：

1 Kit

价格：

询价

Datasheet

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