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Human ATP6V1A Knockdown Cell Line (Wb-Validated) #C63693

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RT-qPCR analysis. HT-1080 cells were infected with ATP6V1A-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.

Western blotting analysis. ATP6V1A protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against ATP6V1A and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

基因名：

ATP6V1A
货号：

C63693
应用：
物种反应性：

规格：

1 Kit

价格：

询价

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Information

Product Name	Human ATP6V1A Knockdown Cell Line (Wb-Validated)
Aliases	ATP6V1A; ATPase H+ Transporting V1 Subunit A; V-ATPase Subunit A; ATP6V1A1; ATP6A1; Vma1; VA68; VPP2; ATPase, H+ Transporting, Lysosomal 70kDa, V1 Subunit A; V-Type Proton ATPase (V-ATPase) Catalytic Subunit A; V-Type Proton ATPase Catalytic Subunit A; Vacuolar Proton Pump Subunit Alpha; ATPase, H+ Transporting, Lysosomal (Vacuolar Proton Pump), Alpha Polypeptide, 70kD, Isoform 1; H+-Transporting ATPase Chain A, Vacuolar (VA68 Type); ATPase, H+ Transporting, Lysosomal, Subunit A1; H(+)-Transporting Two-Sector ATPase, Subunit A; Vacuolar Proton Pump Alpha Subunit 1; Vacuolar ATPase Isoform VA68; V-ATPase 69 KDa Subunit 1; Vacuolar-Type H(+)-ATPase; V-ATPase 69 KDa Subunit; V-ATPase A Subunit 1; EC 3.6.3.14; EC 7.1.2.2; EC 3.6.3; ARCL2D; IECEE3; DEE93; HO68
Background	Gene Name: ATP6V1A NCBI Gene Entry: 523
Storage	Store at liquid nitrogen for 1 year.
Kit Components	1. Human ATP6V1A Knockdown Cell Line (Wb-Validated) 2. Wild-type cell line
Parental Cell Line	Human cell line supplied by the client
Validation Methods	RT-qPCR, Western blotting (WB)
Shipping	Shipped on Dry Ice.
Instructions For Use	This knockdown cell line should be paired with wild-type cell line for use.
Note	This product is for research use only.

Picture

RT-qPCR analysis. HT-1080 cells were infected with ATP6V1A-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. ATP6V1A protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against ATP6V1A and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Citation(0)

基因名：

ATP6V1A
货号：

C63693
应用：
物种反应性：

规格：

1 Kit

价格：

询价

Datasheet

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