Human ATP5F1C Knockdown Cell Line (Wb-Validated) - 产品中心

Human ATP5F1C Knockdown Cell Line (Wb-Validated) #C63811

一键复制产品信息

返回列表

RT-qPCR analysis. HT-1080 cells were infected with ATP5F1C-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.

Western blotting analysis. ATP5F1C protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against ATP5F1C and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

基因名：

ATP5F1C
货号：

C63811
应用：
物种反应性：

规格：

1 Kit

价格：

询价

Information

Picture

Citation(0)

Information

Product Name	Human ATP5F1C Knockdown Cell Line (Wb-Validated)
Aliases	ATP5F1C; ATP Synthase F1 Subunit Gamma; ATP5CL1; ATP5C1; ATP5C; ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, Gamma Polypeptide 1; ATP Synthase Subunit Gamma, Mitochondrial; F-ATPase Gamma Subunit; Mitochondrial ATP Synthase, Gamma Subunit 1; ATP Synthase Gamma Chain, Mitochondrial
Background	Gene Name: ATP5F1C NCBI Gene Entry: 509
Storage	Store at liquid nitrogen for 1 year.
Kit Components	1. Human ATP5F1C Knockdown Cell Line (Wb-Validated) 2. Wild-type cell line
Parental Cell Line	Human cell line supplied by the client
Validation Methods	RT-qPCR, Western blotting (WB)
Shipping	Shipped on Dry Ice.
Instructions For Use	This knockdown cell line should be paired with wild-type cell line for use.
Note	This product is for research use only.

Picture

RT-qPCR analysis. HT-1080 cells were infected with ATP5F1C-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. ATP5F1C protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against ATP5F1C and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Citation(0)

基因名：

ATP5F1C
货号：

C63811
应用：
物种反应性：

规格：

1 Kit

价格：

询价

Datasheet

首页

关于我们

善本标准

产品中心

定制服务

信号通路

促销活动

技术支持

新闻资讯

首页