Human ARIH2 Knockdown Cell Line (Wb-Validated) - 产品中心

Human ARIH2 Knockdown Cell Line (Wb-Validated) #C63813

一键复制产品信息

返回列表

RT-qPCR analysis. HT-1080 cells were infected with ARIH2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.

Western blotting analysis. ARIH2 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against ARIH2 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

基因名：

ARIH2
货号：

C63813
应用：
物种反应性：

规格：

1 Kit

价格：

询价

Information

Picture

Citation(0)

Information

Product Name	Human ARIH2 Knockdown Cell Line (Wb-Validated)
Aliases	ARIH2; Ariadne RBR E3 Ubiquitin Protein Ligase 2; TRIAD1; All-Trans Retinoic Acid Inducible RING Finger; RING-Type E3 Ubiquitin Transferase ARIH2; E3 Ubiquitin-Protein Ligase ARIH2; Protein Ariadne-2 Homolog; ARI2; Ariadne (Drosophila) Homolog 2; Ariadne Homolog 2 (Drosophila); Ariadne Homolog 2; Triad1 Protein; EC 2.3.2.31; ARI-2
Background	Gene Name: ARIH2 NCBI Gene Entry: 10425
Storage	Store at liquid nitrogen for 1 year.
Kit Components	1. Human ARIH2 Knockdown Cell Line (Wb-Validated) 2. Wild-type cell line
Parental Cell Line	Human cell line supplied by the client
Validation Methods	RT-qPCR, Western blotting (WB)
Shipping	Shipped on Dry Ice.
Instructions For Use	This knockdown cell line should be paired with wild-type cell line for use.
Note	This product is for research use only.

Picture

RT-qPCR analysis. HT-1080 cells were infected with ARIH2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. ARIH2 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against ARIH2 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Citation(0)

基因名：

ARIH2
货号：

C63813
应用：
物种反应性：

规格：

1 Kit

价格：

询价

Datasheet

首页

关于我们

善本标准

产品中心

定制服务

信号通路

促销活动

技术支持

新闻资讯

首页